A molecular tool described here allows in one step for specific discrimination among three cryptic freshwater snail species (genus Galba) involved in fasciolosis transmission, a worldwide infectious disease of humans and livestock. The multiplex PCR approach taken targets for each species a distinctive, known microsatellite locus which is amplified using specific primers designed to generate an amplicon of a distinctive size that can be readily separated from the amplicons of the other two species on an agarose gel. In this way, the three Galba species (G. cubensis, G. schirazensis, and G. truncatula) can be differentiated from one another, including even if DNA from all three were present in the same reaction. The accuracy of this new molecular tool was tested and validated by comparing multiplex PCR results with species identification based on sequences at mitochondrial and nuclear markers. This new method is accurate, inexpensive, simple, rapid, and can be adapted to handle large sample sizes. It will be helpful for monitoring invasion of Galba species and for developing strategies to limit the snail species involved in the emergence or re-emergence of fasciolosis.
Bibliographical noteFunding Information:
We thank Nicolás Bonel and anonymous reviewers for their critical review of the manuscript. Fellowships granted by Erasmus Mundus PRECIOSA and Méditerranée Infection supported research stays of PA at the Institute de Recherche pour le Développement, MIVEGEC (Montpellier, France). AV was supported by a grant from IRD (BEST) and ML by a doctoral fellowship from University of Montpellier and a post-doctoral grant from Labex CeMeb . This study was financially supported by University of Montpellier , IRD , and CNRS .
© 2018 Elsevier B.V.
- Infectious disease
- Multiplex PCR