Bioensayos ecotoxicológicos en nanopartículas de puntos cuánticos con la microalga Pseudokirchneriella subcapitata

Translated title of the contribution: Ecotoxicological bioassays in quantum dots nanoparticles with the microalgae pseudokirchneriella subcapitata

Ronald Huarachi-Olivera, Ursulo Yapo, Alex Dueñas-Gonza, Gonzalo Soclle-Huamantuma, Diana Sánchez-Sarmiento, Margiht Romero-Ugarte, Antonio Mateo Lazarte Rivera, Mario Esparza

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Quantum dots (QDs) nanoparticles are increasingly being used for electronics devices, solar energy generation and medical imaging applications. While the new properties of nanoparticles are more and more studied, little is known about their interactions with aquatic organisms. As a primary producer, phytoplankton serves as an important component of the aquatic ecosystem. According to our bibliographic search, no reports were found on the evaluation of ecotoxicity of QDs in Pseudokirchneriella subcapitata. In this study, QDs were evaluated using ecotoxicological bioassays (cellular toxicity models and potential indicators of the impact in aquatic ecosystems) with the microalgae P. subcapitata. With ecotoxicity concentrations of 0, 1, 2, 3 and 4 nM of QDs with P. subcapitata at 96 h exposure a mean inhibitory concentration of growth (IC50) of the microalgae P. subcapitata IC50 = 1,94 nM of QDs(a) was obtained for orange fluorescence; IC50 = 1.68 nM of QDs(r) for red fluorescence, and IC50 = 2.03 nM of QDs(v) for green fluorescence, observing the following order of sensitivity: QDs (r) ˃ QDs (a) ˃ QDs (v). The toxicity of the QDs are evident by the type of fluorescence detected in the standard solution 4 M of QDs and 10-1M. The images of confocal microscopy of fluorescence showed adhesions of QDs in microalgal cells until low concentrations with high growth rates of P. subcapitata at concentrations of 1 nM compared to concentrations of 10-1 M of QDs nanoparticles with low growth rates.

Translated title of the contributionEcotoxicological bioassays in quantum dots nanoparticles with the microalgae pseudokirchneriella subcapitata
Original languageSpanish
Pages (from-to)757-769
Number of pages13
JournalRevista Internacional de Contaminacion Ambiental
Volume35
Issue number3
DOIs
StatePublished - 2019

Bibliographical note

Funding Information:
The first author thanks the University of Antofagasta, Chile, for a postgraduate scholarship in Biotechnology, the Institute of Antofagasta, Chile, for project MEM 2015 ATI15-02/ATI15-03, and the Directorate of Research Management of the University of Antofagasta for the Semillero Project 5302-DGI UA. The authors are grateful to Dr. J.M. Pérez-Donoso for his scientific contribution to this work during their Master’s Program in Biotechnology in the Laboratory of Bionanotechnology and Microbiology of the Andrés Bello University, Santiago, Chile.

Funding Information:
The first author thanks the University of Antofagasta, Chile, for a postgraduate scholarship in Biotechnology, the Institute of Antofagasta, Chile, for project MEM 2015 ATI15-02/ATI15-03, and the Directorate of Research Management of the University of Antofagasta for the Semillero Project 5302-DGI UA. The authors are grateful to Dr. J.M. P?rez-Donoso for his scientific contribution to this work during their Master?s Program in Biotechnology in the Laboratory of Bionanotechnology and Microbiology of the Andr?s Bello University, Santiago, Chile.

Publisher Copyright:
© 2019, Centro de Ciencias de la Atmosfera, UNAM. All rights reserved.

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