Differential expression of circulating micro-rnas in patients with active and latent tuberculosis

José Yareta, Marco Galarza, Silvia Capristano, Oscar Pellón, César Sánchez, Jorge Andres Ballon Echegaray, Heinner Guio

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: To analyze the differential expression of miR-21, miR-29a, miR-99b and miR-155 in serum samples from patients with latent tuberculosis (TB) and active TB compared to healthy controls. Materials and Methods: We used 28 serum samples (9 with active TB, 10 with latent TB and 9 healthy con-trols) for the analysis of gene expression by RT-qPCR with Primers and TaqMan probes. The differential expression was calculated by the Livak method using a normalizing gene (RNU-48). Results: Overex-pression of miR-155 was found in people with latent tuberculosis, compared to healthy controls (0.63 vs. 0.01; p value = 0.032). Conclusion: The miR-155 could be considered a biomarker to differentiate latent TB from active disease. Studies with larger sample sizes are required to corroborate the findings.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalRevista Peruana de Medicina de Experimental y Salud Publica
Volume37
Issue number1
DOIs
StatePublished - 2020

Bibliographical note

Publisher Copyright:
© 2020, Instituto Nacional de Salud. All rights reserved.

Keywords

  • Latent Tuberculosis
  • MicroRNAs
  • Peru (source: MeSH NLM)
  • Tuberculosis

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