Abstract
Cantua volcánica (Polemoniaceae) is a species endemic to Arequipa, Peru. This plant, which has an ornamental potential, is endangered by habitat loss due to anthropogenic pressures and climate change. A protocol was developed for in vitro micropropagation using nodal explants, allowing its massive propagation in vitro. For the establishment stage, 1/2 MS was determined to be the best culture medium. In the proliferation stage, the growth regulator balance that showed the highest survival rate was MS + IBA 1 ppm + BA 0.5 ppm. Different combinations of NAA-AIB were tested for rooting; MS + NAA 0.005 ppm + IBA 0.005 ppm was determined as the best medium. In the last stage, 65% of in vitro plants were acclimatized using vermiculite as substrate and irrigating with 50% Hoagland mineral solution.
| Translated title of the contribution | Establishment of an in vitro micropropagation protocol for cantua volcánica (Polemoniaceae): A potential ornamental plant of Peru |
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| Original language | Spanish |
| Pages (from-to) | 203-208 |
| Number of pages | 6 |
| Journal | Idesia |
| Volume | 36 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jul 2018 |
Bibliographical note
Funding Information:Financial support was provided by National Universidad Nacional de San Agustín de Arequipa through Cienci Activa with contract N° TT-0016-2016. We sincerely thank Mg. Luis Martínez Manchego of National University of San Agustin for his guidance in the statistical analysis of this investigation. We also recognize the lab assistance of Patricia Viveros Flores and Ana Caldas Araujo.
Publisher Copyright:
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