Transcriptomic changes induced by drought stress in hardneck garlic during the bolting/bulbing stage

Xiangjun Zhou, Jorge Alberto Condori-Apfata, Xiaoqin Liu, Sandro Jhonatan Condori Pacsi, Maria Valderrama Valencia, Cankui Zhang

Research output: Contribution to journalArticlepeer-review

Abstract

Garlic (Allium sativum L.) is an economically important, monocotyledonous plant with a strong taste and odor. Drought stress adversely affects its growth, development, and yield, particularly during the bolting/bulbing stage. Herein we performed RNA-seq to assess transcriptomic changes induced by drought stress in bolting/bulbing hardneck garlic plants (Purple Glazer). We observed that drought stress significantly reduced photosynthesis rate, fresh weight, and leaf water content. Transcriptomic analysis of garlic leaves under normal conditions and drought stress led to the identification of 5215 differentially expressed genes (2748 up-and 2467 downregulated). The upregulated DEGs were primarily involved in “biological process”, “metabolic process”, “oxidation-reduction process”, carbohydrate and lipid metabolism, and “proteolysis”, whereas the downregulated DEGs were mainly involved in “biological process” and metabolism of various molecules. In addition, genes encoding abscisic acid biosynthetic and catabolic enzymes, heat shock proteins, and E3 ubiquitin ligases were significantly altered by drought stress, indicating involvement in drought tolerance. A further comparison with the DEGs related to salinity stress-treated garlic revealed 867 and 305 DEGs with a similar and reverse expression alteration tendency, respectively.

Original languageEnglish
Article number246
JournalAgronomy
Volume11
Issue number2
DOIs
StatePublished - Feb 2021

Bibliographical note

Funding Information:
Funding: This work was supported by Nexus Crops CRISPR and Phenomics: Individual and Institutional Capacity Building for Crop Science at Universidad Nacional de San Agustin (UNSA), Peru and the USDA National Institute of Food and Agriculture (Hatch project 1007567).

Funding Information:
This work was supported by Nexus Crops CRISPR and Phenomics: Individual and Institutional Capacity Building for Crop Science at Universidad Nacional de San Agustin (UNSA), Peru and the USDA National Institute of Food and Agriculture (Hatch project 1007567). We thank Zhangjun Fei for his help with bioinformatics analysis. We are grateful to Throckmorton Purdue Agricultural Center for helping us grow garlic.

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Bolting/bulbing
  • Drought stress
  • Garlic
  • Hardneck
  • RNA-seq

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