Yacon fructans (Smallanthus sonchifolius) extraction, characterization and activation of macrophages to phagocyte yeast cells

Larry Ladislao Ramos Paredes, Fhernanda R. Smiderle, Arquimedes P. Santana-Filho, Alexandre Kimura, Marcello Iacomini, Guilherme L. Sassaki

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Yacon (Smallanthus sonchifolius) originates from the Andean region and has spread across South America, Europe and Japan. In contrast with most roots, yacon stores its carbohydrates in fructooligosaccharides (FOS) and contains approximately 37% of FOS in its root dry matter. Aqueous extracts of yacon were characterized through TLC, methylation, NMR, and ESI–MS. FOS of yacon showed as linear fructooligosaccharides containing almost exclusively (2 → 1)-linked β-fructofuranosyl units, with terminal α-glucopyranosyl and β-fructofuranosyl units. ESI–MS analyses indicated a wide degree of polymerization (DP) ranging from 2 to 10. The effect of the isolated FOS on non-specific immune activity by THP-1 cells was evaluated through phagocytic activity against heat-killed yeast (Saccharomices cerevisiae). The stimulant effect of yacon FOS was dose- and time-dependent, showing results more effective than branched FOS observed in previous studies. The results reinforce the use of linear yacon FOS as immunomodulators.

Original languageEnglish
Pages (from-to)1074-1081
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume108
DOIs
StatePublished - Mar 2018
Externally publishedYes

Bibliographical note

Funding Information:
The authors would like to thank the Brazilian funding agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) , Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) , Fundação Araucária and Dr. Lucimara Cordeiro for kindly supplying yacon. The authors also wish to thank the UFPR NMR Centre.

Funding Information:
The authors would like to thank the Brazilian funding agencies Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior (CAPES), Funda??o Arauc?ria and Dr. Lucimara Cordeiro for kindly supplying yacon. The authors also wish to thank the UFPR NMR Centre.

Publisher Copyright:
© 2017 Elsevier B.V.

Keywords

  • Fructooligosaccharides
  • Phagocytic activity
  • Smallanthus sonchifolius
  • THP-1 cells

Fingerprint

Dive into the research topics of 'Yacon fructans (Smallanthus sonchifolius) extraction, characterization and activation of macrophages to phagocyte yeast cells'. Together they form a unique fingerprint.

Cite this