A new multiplex PCR assay to distinguish among three cryptic Galba species, intermediate hosts of Fasciola hepatica

Pilar Alda, Manon Lounnas, Antonio Alejandro Vázquez, Rolando Ayaqui Flores, Manuel Calvopiña, Maritza Celi-Erazo, Robert T. Dillon, Philippe Jarne, Eric S. Loker, Flavia Caroll Muñiz Pareja, Jenny Muzzio-Aroca, Alberto Orlando Nárvaez, Oscar Noya, Luiggi Martini Robles, Richar Rodríguez-Hidalgo, Nelson Uribe, Patrice David, Jean Pierre Pointier, Sylvie Hurtrez-Boussès

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

12 Citas (Scopus)

Resumen

A molecular tool described here allows in one step for specific discrimination among three cryptic freshwater snail species (genus Galba) involved in fasciolosis transmission, a worldwide infectious disease of humans and livestock. The multiplex PCR approach taken targets for each species a distinctive, known microsatellite locus which is amplified using specific primers designed to generate an amplicon of a distinctive size that can be readily separated from the amplicons of the other two species on an agarose gel. In this way, the three Galba species (G. cubensis, G. schirazensis, and G. truncatula) can be differentiated from one another, including even if DNA from all three were present in the same reaction. The accuracy of this new molecular tool was tested and validated by comparing multiplex PCR results with species identification based on sequences at mitochondrial and nuclear markers. This new method is accurate, inexpensive, simple, rapid, and can be adapted to handle large sample sizes. It will be helpful for monitoring invasion of Galba species and for developing strategies to limit the snail species involved in the emergence or re-emergence of fasciolosis.

Idioma originalInglés
Páginas (desde-hasta)101-105
Número de páginas5
PublicaciónVeterinary Parasitology
Volumen251
DOI
EstadoPublicada - 15 feb. 2018

Nota bibliográfica

Publisher Copyright:
© 2018 Elsevier B.V.

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