Biochemical and hemostatic description of a thrombin-like enzyme TLBro from Bothrops roedingeri snake venom

Augusto Vilca-Quispe, Aldo Alvarez-Risco, Mauricio Aurelio Gomes Heleno, Emilio Alberto Ponce-Fuentes, Corina Vera-Gonzales, Herly Fredy Enrique Zegarra-Aragon, Juan Luis Aquino-Puma, María Elena Talavera-Núñez, Shyla Del-Aguila-Arcentales, Jaime A. Yáñez, Luis Alberto Ponce-Soto

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Resumen

Objective: The current study’s objective is to characterize a new throm-bin-like enzyme called TLBro that was obtained from Bothrops roedingeris snake from a biochemical and hemostatic perspective. Methodology: One chromatographic step was used to purify it, producing the serine protease TLBro. Molecular mass was estimated by SDS-PAGE to be between reduced and unreduced by 35 kDa. Tryptic peptide sequencing using Swiss Prot provided the complete amino acid sequence. Expasy.org by conducting a search that is limited to Crotalinae snake serine proteases and displaying a high degree of amino acid sequence. Results: Ser (182) is inhibited by phenylmethylsulfonyl fluoride (PMSF), and TLBro demonstrated the presence of Asp (88) residues. It also deduced the positions of His (43) and Ser (182) in the set of three coordinated amino acids in serine proteases. It was discovered that this substrate had high specificity for BANA, Michaelis-Menten behavior with KM 0 point85 mM and Vmax 1 point89 nmoles -NA/L/min, and high stability between temperatures (15 to 70°C) and pHs (2 point0 to 10 point0). According to doses and incubation times, TLBro degraded fibrin preferentially on the B-chain; additionally, its activities were significantly diminished after preincubation with divalent ions (Zn2 and Cd2). When incubated with PMSF, a particular serine protease inhibitor, enzymatic activities and platelet aggregation were inhibited. Conclusion: The findings revealed distinct structural and functional differences between the serine proteases, adding to the information and assisting in the improvement of the structure-function relationship.

Idioma originalInglés
Número de artículo1217329
PublicaciónFrontiers in Chemistry
Volumen11
DOI
EstadoPublicada - 2023

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Copyright © 2023 Vilca-Quispe, Alvarez-Risco, Gomes Heleno, Ponce-Fuentes, Vera-Gonzales, Zegarra-Aragon, Aquino-Puma, Talavera-Núñez, Del-Aguila-Arcentales, Yáñez and Ponce-Soto.

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