Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

Hugo Canahua-Loza; Paul Huanca-Zuñiga; Belinda Chavez; Leslie Canahua-Sosa; Jonathan Almirón; Danny Tupayachy-Quispe

doi:10.4028/p-0z37mj

Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

Hugo Canahua-Loza, Paul Huanca-Zuñiga, Belinda Chavez, Leslie Canahua-Sosa, Jonathan Almirón, Danny Tupayachy-Quispe

Resultado de la investigación: Capítulo del libro/informe/acta de congreso › Capítulo › revisión exhaustiva

Resumen

In this study, the degree of acetylation (DA) and degree of deacetylation (DDA) were determined by Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and their viability was evaluated by the cytocompatibility test with L132 epithelial cells, from samples of chitin and chitosan extracted of Cryphiops caementarius. FT-IR measurements resulted in the successful determination of DA and DDA. Particularly the results showed that, the chitosan CHT-C has the highest DDA (93%), while viability tests employed to determine the cytotoxicity of chitin and chitosan showed that the cell survival rate of CH-C and CHT-C are 130% and 75% respectively while commercial CHT has a rate of 80% approximately, demonstrating that the CH-C (chitin) a favorable behavior against L132 cell.

Idioma original	Inglés
Título de la publicación alojada	Materials Science Forum
Editorial	Trans Tech Publications Ltd
Páginas	15-20
Número de páginas	6
DOI	https://doi.org/10.4028/p-0z37mj
Estado	Publicada - 2022

Serie de la publicación

Nombre	Materials Science Forum
Volumen	1060 MSF
ISSN (versión impresa)	0255-5476
ISSN (versión digital)	1662-9752

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Publisher Copyright:
© 2022 Trans Tech Publications Ltd, Switzerland.

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Canahua-Loza, H., Huanca-Zuñiga, P., Chavez, B., Canahua-Sosa, L., Almirón, J., & Tupayachy-Quispe, D. (2022). Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field. En Materials Science Forum (pp. 15-20). (Materials Science Forum; Vol. 1060 MSF). Trans Tech Publications Ltd. https://doi.org/10.4028/p-0z37mj

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abstract = "In this study, the degree of acetylation (DA) and degree of deacetylation (DDA) were determined by Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and their viability was evaluated by the cytocompatibility test with L132 epithelial cells, from samples of chitin and chitosan extracted of Cryphiops caementarius. FT-IR measurements resulted in the successful determination of DA and DDA. Particularly the results showed that, the chitosan CHT-C has the highest DDA (93%), while viability tests employed to determine the cytotoxicity of chitin and chitosan showed that the cell survival rate of CH-C and CHT-C are 130% and 75% respectively while commercial CHT has a rate of 80% approximately, demonstrating that the CH-C (chitin) a favorable behavior against L132 cell.",

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Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field. / Canahua-Loza, Hugo; Huanca-Zuñiga, Paul; Chavez, Belinda et al.

Materials Science Forum. Trans Tech Publications Ltd, 2022. p. 15-20 (Materials Science Forum; Vol. 1060 MSF).

Resultado de la investigación: Capítulo del libro/informe/acta de congreso › Capítulo › revisión exhaustiva

TY - CHAP

T1 - Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

AU - Canahua-Loza, Hugo

AU - Huanca-Zuñiga, Paul

AU - Chavez, Belinda

AU - Canahua-Sosa, Leslie

AU - Almirón, Jonathan

AU - Tupayachy-Quispe, Danny

PY - 2022

Y1 - 2022

N2 - In this study, the degree of acetylation (DA) and degree of deacetylation (DDA) were determined by Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and their viability was evaluated by the cytocompatibility test with L132 epithelial cells, from samples of chitin and chitosan extracted of Cryphiops caementarius. FT-IR measurements resulted in the successful determination of DA and DDA. Particularly the results showed that, the chitosan CHT-C has the highest DDA (93%), while viability tests employed to determine the cytotoxicity of chitin and chitosan showed that the cell survival rate of CH-C and CHT-C are 130% and 75% respectively while commercial CHT has a rate of 80% approximately, demonstrating that the CH-C (chitin) a favorable behavior against L132 cell.

AB - In this study, the degree of acetylation (DA) and degree of deacetylation (DDA) were determined by Fourier Transform Infrared Spectroscopy (FT-IR) analysis, and their viability was evaluated by the cytocompatibility test with L132 epithelial cells, from samples of chitin and chitosan extracted of Cryphiops caementarius. FT-IR measurements resulted in the successful determination of DA and DDA. Particularly the results showed that, the chitosan CHT-C has the highest DDA (93%), while viability tests employed to determine the cytotoxicity of chitin and chitosan showed that the cell survival rate of CH-C and CHT-C are 130% and 75% respectively while commercial CHT has a rate of 80% approximately, demonstrating that the CH-C (chitin) a favorable behavior against L132 cell.

KW - Chitin

KW - chitosan

KW - Cryphiops camentarius

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U2 - 10.4028/p-0z37mj

DO - 10.4028/p-0z37mj

M3 - Capítulo

AN - SCOPUS:85130593845

T3 - Materials Science Forum

SP - 15

EP - 20

BT - Materials Science Forum

PB - Trans Tech Publications Ltd

ER -

Biological Characterization of Different Samples of Chitin and Chitosan for their Application on Biomedical Field

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